Journal: Hormones & Cancer

Article Title: Estrogen Receptor-beta Mediates the Protective Effects of Aromatase Induction in the MMTV-Her-2/neu x Aromatase Double Transgenic Mice

doi: 10.1007/s12672-011-0083-5

Figure Lengend Snippet: The expression of angiogenic factors is decreased in MMTV-Her-2/neu x aromatase double transgenic mice. RNA isolated from the MMTV-Her-2/neu x aromatase double transgenic mice, parental strains, and FVB wild-type mice was subjected to real-time RT-PCR to examine the expression of BRCA1, FAS, and its ligand (a), a panel of angiogenic factors (b), and a panel of apoptosis-related genes (c). Note the strong induction of BRCA1 mRNA and decrease in Angio 1, TIE1, and TIE2 in the MMTV-Her-2/neu x aromatase mice compared to the parental strain MMTV-Her-2/neu (*P < 0.01)

Article Snippet: Pathway-specific RT-PCR arrays (RT 2 Profiler™) were obtained from SuperArray (Frederick, MD).

Techniques: Expressing, Transgenic Assay, Isolation, Quantitative RT-PCR

Journal: PLoS ONE

Article Title: The Bile Acid Sensor FXR Is Required for Immune-Regulatory Activities of TLR-9 in Intestinal Inflammation

doi: 10.1371/journal.pone.0054472

Figure Lengend Snippet: (A–B) Quantitative RT-PCR of FXR and TNFα genes was carried out on RNA purified from CD14 positive cells derived PBMC stimulated ex vivo with TLRs agonists as described in the . Data are mean ± SE of 3 experiments carried out in triplicate. *P<0.05 versus not treated cells. (C) Specificity of CpG effect. PCR array analysis showing the relative mRNA expression of various nuclear receptors on CD14 + derived PBMC stimulated ex vivo with TLR9 agonist CpG. (D) Quantitative RT-PCR of FXR was carried out on RNA purified from spleen-derived monocytes isolated from TLR9 +/+ and TLR9 −/− mice stimulated ex vivo with CpG. Data are mean of ± SE of 4 mice. *P<0.05 versus TLR9 +/+ not treated cells.

Article Snippet: 25 ng cDNA was pipetted in each well of a 96 well PCR array plate (Human Nuclear Receptors and Coregulators RT2 Profiler TM PCR Array - http://www.sabiosciences.com/rt_pcr_product/HTML/PAHS-056A.html - Superarray Bioscience, Frederick,MD, USA) and amplified following the manual instructions.

Techniques: Quantitative RT-PCR, Purification, Derivative Assay, Ex Vivo, Expressing, Isolation

Journal: PLoS ONE

Article Title: Upregulation of PTEN in Glioma Cells by Cord Blood Mesenchymal Stem Cells Inhibits Migration via Downregulation of the PI3K/Akt Pathway

doi: 10.1371/journal.pone.0010350

Figure Lengend Snippet: Effect of hUCBSC on glioma cells after co-culture (cDNA microarray results of PI3K-AKT pathway).

Article Snippet: For real time polymerase chain reaction (RT-PCR) analysis and RT-PCR-based microarray analysis (RT 2 Profiler PCR Array, SuperArray, Frederick, MD), total RNA was isolated from control and hUCBSC-treated cancer cells.

Techniques: Microarray